40882
Fluka
JC-1
BioChemika, ≥90% (HPCE)
| Synonym: | CBIC2(3), 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide, 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide |
| CAS Number: | 3520-43-2 |
| Linear Formula: | C25H27Cl4IN4 |
| Molecular Weight: | 652.23 |
| MDL number: | MFCD00188475 |
| PubChem Substance ID: | 24865598 |
Description
| Application | A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density. |
Properties
| product line | BioChemika |
| assay | ≥90% (HPCE) |
| fluorescence | λex 520 nm; λem 596 nm in 0.1 M Tris pH 8.0 |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
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References
| reference | L.B. Chen and S.T. Smiley, W.T. Mason, ed. Fluorescent and Luminescent Probes for Biological Activity, (1993), 124 |
| M. Reers et al., Mitochondrial membrane potential monitored by JC-1 dye. Meth. Enzymol. 260, 406-417, (1995) Abstract | |
| M. Reers et al., Mitochondrial membrane potential monitored by JC-1 dye. Meth. Enzymol. 260, 406-417, (1995) Abstract | |
| S.T. Smiley et al., Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1. Proc. Natl. Acad. Sci. U. S. A. 88, 3671-3675, (1991) Abstract |
